ABCC11 Rat Monoclonal Antibody [Clone ID: M8I-74]

CAT#: AM31991SU-N

ABCC11 rat monoclonal antibody, clone M8I-74, Supernatant

Specifications

Product Data

• Clone Name: M8I-74
• Applications: IF, IHC, WB
• Recommended Dilution: Western blotting: Use at 1/20-1/50 and anti-Rat-HRP.
  Immunocytochemistry: Use at 1/20-1/50 on Acetone Fixed Cytospin preparations.
  Immunohistochemisty on Frozen Sections: 1/20 on Acetone Fixed Frozen sections can be followed by incubation with Rabbit anti-Rat Ig (1/25) and a monoclonal Rat APAAP complex (1/50). Alternatively, after incubation with M8I-74 (use 1/20) and washing, slides can be incubated with biotinylated Rabbit anti-Rat Ig (1/100) and streptavidin conjugated to Horseradish Peroxidase (1/500).
• Reactivities: Human
• Host: Rat
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A bacterial fusion protein of Human MRP8, containing amino acids 1-83 of the protein.
• Specificity: This Monoclonal antibody clone M8I-74 reacts with an internal epitope of MRP8 (ABCC11), an approximately 150 kD transmembrane protein that is related to the multidrug resistance protein MRP1.
• Formulation: State: Supernatant
  State: Serum Free Culture Supernatant
  Stabilizer: 0.7% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Storage: Store antibody undiluted at 2-8°C.
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 85320 Human
• Background: ABCC11 belongs to the ABC transporter family, conjugate transporter (TC 3.A.1.208) subfamily. It contains two ABC transmembrane type-1 domains and two ABC transporter domains.
  ABCC11 participates in physiological processes involving bile acids, conjugated steroids and cyclic nucleotides and enhances the cellular extrusion of cAMP and cGMP. It also stimulates the ATP-dependent uptake of a range of physiological and synthetic lipophilic anions, including the glutathione S-conjugates leukotriene C4 and dinitrophenyl S-glutathione, steroid sulfates such as dehydroepiandrosterone 3-sulfate (DHEAS) and estrone 3-sulfate, glucuronides such as estradiol 17-beta-D-glucuronide (E(2)17betaG), the monoanionic bile acids glycocholate and taurocholate, and methotrexate. It probably functions to secrete earwax.
• Synonyms: EWWD; MRP8; OTTHUMP00000164191; WW