ABCB4 Mouse Monoclonal Antibody [Clone ID: P3II-26]

CAT#: AM32042SU-N

ABCB4 mouse monoclonal antibody, clone P3II-26, Supernatant

Specifications

Product Data

• Clone Name: P3II-26
• Applications: IF, IHC, WB
• Recommended Dilution: Western blotting.
  Immunocytochemistry: Use 1/20-1/50 on acetone fixed cytospin preparations.
  Immunohistochemistry on Acetone Fixed Frozen Sections: 1/20 followed by incubation with Rabbit anti-Mouse IgG and a monoclonal Mouse APAAP complex.
  P3II-26 does not work very well on formaldehyde-fixed paraffin-embedded human tissues.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Fusion protein consisting of Gluthathione S Transferase and a fragment of MDR3 P-Glycoprotein comprising amino acid 629-692.
• Specificity: This Monoclonal antibody P₃II-26 reacts with an internal epitope of MDR3 P-Glycoprotein.
  P₃II-26 does not cross-react with the Human MDR1 P-Glycoprotein.
• Formulation: State: Supernatant
  State: Serum Free Culture Supernatant
  Stabilizer: 0.7% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5244 Human
• Background: The membrane-associated protein encoded ABCB4 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function.
• Synonyms: Multidrug resistance protein 3, P-glycoprotein 3, PGY3