Egf Mouse Monoclonal Antibody [Clone ID: F5]

CAT#: AM32085SU-N

Egf mouse monoclonal antibody, clone F5, Supernatant

Specifications

Product Data

• Clone Name: F5
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Spot Blots.
  Immunohistochemistry on Fixed Frozen Sections: 1/20.
  Immunohistochemistry on Paraffin Sections of Salivary glands (see Protocols for more details.)
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: EGF coupled on BSA and tetanus toxoide
• Specificity: The antibody reacts with Mouse EGF in ELISA (10 ng detectable) and in Spot Blots (1 ng detectable).
  In Immunohistochemistry the antibody reacts with formalin fixed and paraffin embedded Mouse salivary glands.
  It also reacts with Human Brunner's glands (presumably with urogastron).
• Formulation: State: Supernatant
  State: Tissue Culture Supernatant
  Stabilizer: 1.0% BSA
  Preservative: 20 mM Sodium Azide
• Concentration: lot specific
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  
• Stability: Shelf life: one year from despatch.
• Gene Name: epidermal growth factor
• Database Link:
  Entrez Gene 13645 Mouse
• Background: Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein.
  Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.
• Synonyms: Urogastrone, Epidermal growth factor, URG, HOMG4