Lbp Rat Monoclonal Antibody [Clone ID: M330-19]

CAT#: AM32123PU-N

Lbp rat monoclonal antibody, clone M330-19, Liquid

Specifications

Product Data

• Clone Name: M330-19
• Applications: ELISA, FN, NEUT, WB
• Recommended Dilution: Western Blot: It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1/10.
  Immunoassay.
  Functional studies in vitro and in vivo aimed at blocking LBP.
  For neutralization of biological activity dilutions have to be made according to the amounts LBP to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
  
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity:

  The monoclonal antibody M330-19 reacts highly specific with mouse natural and recombinant LBP. The antibody is a type I antibody blocking the LPS binding to LBP.

• Formulation: PBS
  State: Liquid
  State: Liquid purified Ig fractions.
  Stabilizer: 0,1% BSA
  Preservative: 0,02% sodium azide
• Concentration: lot specific
• Storage:

  Store the antibody undiluted at 2-8°C.

• Stability: Shelf life: one year from despatch.

• Gene Name: lipopolysaccharide binding protein
• Database Link:
  Entrez Gene 16803 Mouse
• Background:

  LPS binding protein (LBP) is an approximately 60 kDa acute phase protein that is produced by hepatocytes. This protein strongly binds to LPS and has been shown to play an important role in the handling of LPS by the host. A number of functions of LBP have been reported. First, LBP transfers LPS to the LPS receptor CD14 on mononuclear phagocytes, leading to an 100-1,000-fold increased sensitivity of the cells to LPS. Furthermore, LBP can enhance the response of CD14 negative cells by acceleration of LPS binding to soluble CD14, a complex that stimulates these cells. Next, LBP transfers LPS into High Density Lipoprotein (HDL), which effectively neutralizes its biological potency. LBP was demonstrated to protect mice from septic shock caused by LPS or gram negative bacteria.

• Synonyms: Lipopolysaccharide-binding protein