CD21 (CR2) Mouse Monoclonal Antibody [Clone ID: 4C5]

CAT#: AM32139PU-N

CD21 (CR2) mouse monoclonal antibody, clone 4C5, Ig Fraction

Specifications

Product Data

• Clone Name: 4C5
• Applications: IF, IHC
• Recommended Dilution:

  Immunofluorescence.
  Flow Cytometry: 1/50.
  Immunohistochemistry on (Aceton-Fixed) Frozen Sections and Cytospin preparations. Use ~1/20-1/50 dilution (depends on technique).

• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: A BALB/c mouse was immunized subcutaneously in its footpads with fragments of a Human tonsil.
  After fusing the lymphocytes from the popliteal lymph nodes of this mouse with murine SP2/0 myeloma cells
• Specificity: This Monoclonal antibody 4C5 with the CD21 (140kD) molecule, expressed (moderate) on mature B-cells and (at high density) on follicular dendritic cells (FDC).
  The antibody producing cells were selected on their immunohistochemical staining pattern.
  Later on the antibody was biochemi-cally analysed and revealed to recognize the CD21 molecule (140kD) by Immunoprecipitation procedures.
  4C5 shows moderate labeling of mature B-cells and strong labeling of FDC's on acetone-fixed cryostat sections, air-dried and acetone-fixed cells and Immunofluorescence and Immuno-peroxidase tests.
• Formulation: State: Ig Fraction
  State: Liquid Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Storage:

  Store the antibody undiluted at 2-8°C for Three months or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 145 kDa
• Gene Name: complement component 3d receptor 2
• Database Link:
  Entrez Gene 1380 Human
• Background: CD21 is a type I integral membrane glycoprotein that serves as a receptor for the C3d complement fragment and for the Epstein-Barr virus. It plays a role in B cell activation and proliferation and undergoes phosphorylation after B cell activation with phorbol esters. CD21 is expressed on mature B cells, follicular Dendritic cells, pharyngeal and cervical epithelial cells and a subset of thymocytes. The adaptive immune response is tightly regulated to limit responding cells in an antigen-specific manner. On B cells, co-receptors CD21/CD19 modulate the strength of B cell Ag receptor (BCR) signals, thereby influencing cell fate. Complement receptor (CR) type 2 (CR2/ CD21) is normally expressed during the immature and mature stages of B cell development. In association with CD19, CR21 plays an important role in enhancing mature B cell responses to foreign antigen.
• Synonyms: Complement receptor type 2, CR2, C3DR, C3d receptor, EBV Receptor, Dendritic Cell Marker