Melanoma (25-110 kDa) Mouse Monoclonal Antibody [Clone ID: NKI/C3]

CAT#: AM32146SU-N

Melanoma (25-110 kDa) mouse monoclonal antibody, clone NKI/C3, Ascites

Specifications

Product Data

• Clone Name: NKI/C3
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Paraffin Sections.
  Standard ABC technique.
  Working Dilution: 1/10-1/20.
  Incubation Time: 60 minutes at 25°C.
  Positive Control: Tonsil.
  Staining Pattern: Cytoplasmic and membrane.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Smooth plasma membrane fraction of MeWo cells.
• Specificity:

  This antibody clone NKI/C-3 recognises a heterogeneous 25-110 kD glycoprotein that is located mainly in the innerside of membranes of cytoplasmic vesicles in melanoma cells.
  The antigen has a 25 kD unglycosylated precursor in formalin fixed and paraffin-embedded tissue sections.
  Cross-reactivity: The antibody reacts with some mucus producing tumors, carcinoids, carcinomas of the thyroid, mast cells, histiocytes in tumor regions and with cells with secretory functions such as salivary glands, bronchial glands, sweat glands, pancreas and prostate.

• Formulation: 50mM PBS, pH 7.4
  State: Ascites
  State: Liquid diluted Ascites
  Stabilizer: 1% BSA
  Preservative: 15mM Sodium Azide
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Synonyms: Melanoma marker