High Density Lipoprotein / HDL (HDL2, HDL3, APO-A1) Mouse Monoclonal Antibody [Clone ID: 1C5]

CAT#: AM32208PU-N

High Density Lipoprotein / HDL (HDL2, HDL3, APO-A1) mouse monoclonal antibody, clone 1C5, Purified

Specifications

Product Data

• Clone Name: 1C5
• Applications: ELISA, IHC
• Recommended Dilution: ELISA: Use ~1/1000 dilution.
  The antibody can be used for HDL depletion of Human plasma, for detection of HDL in plasma either in combination with other mabs by using Sandwich ELISA or in Competitive ELISA.
  Immunohistology on Frozen Sections: Use ~1/100 dilution.
  The antibody is also useful for detection of HDL in tissue sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: The antibody reacts with High Density Lipoproteins (HDL2, HDL3, Apo A1) of Human plasma. No cross reactions with other plasma proteins were detected.
  This antibody increases the specific binding of HDL to Rat and Human hepatocytes and the efflux of Cholesterol from hepatocytes prelabelled with ¹⁴C-Acetate.
• Formulation: 10 mM Sodium Phosphate, 100 mM Sodium Chloride, pH 8.0
  State: Purified
  State: Lyophilized purified Ig fraction
  Preservative: 1 mM Sodium Azide and 0.1% (w/v) Mannitol
• Reconstitution Method: Restore with 1 ml sterile water and add preservative if preferred.
• Concentration: lot specific
• Purification: Sodium Sulfate and DEAE-Cellulose Chromatography
• Storage:
  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: HDL is a cholesterol carrier protein that acts as scavenger of tissue cholesterol. It is important in cholesterol efflux from tissues, and involved in the return of cholesterol from the periphery to the liver for removal as bile acids.