Collagen V (COL5A1) Mouse Monoclonal Antibody [Clone ID: MC5-HA]

CAT#: AM32236PU-N

Collagen V (COL5A1) mouse monoclonal antibody, clone MC5-HA, Purified

Specifications

Product Data

• Clone Name: MC5-HA
• Applications: ELISA, IHC, R
• Recommended Dilution: Radioimmunoassay.
  ELISA: 1/1000.
  Immunohistochemistry on frozen sections: 1/20.
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Specificity: The antibody reacts with native Human as well as heat denatured Human Collagen type V in RIA, ELISA and immunoblot assays. The antibody does not react with Collagen types I, II, III and IV. Human plasma proteins do not interfere with binding to collagen.
• Formulation: State: Purified
  State: Lyophilized purified Ascites
• Reconstitution Method: Restore with 1 ml sterile water. Add preservative if preferred.
• Purification: PEG6000 precipitation + Sepharose 6B gel filtration
• Storage:

  Store the antibody undiluted at 2-8°C.

• Stability: Shelf life: one year from despatch.

• Gene Name: collagen type V alpha 1
• Database Link:
  Entrez Gene 1289 Human
• Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure.
  Type V Collagen is a member of group I Collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V Collagen binds to DNA, heparan sulfate, Thrombospondin, Heparin, and Insulin. Collagen V is composed of several subunits as follows: trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Type V Collagen is closely related to type XI Collagen and it is possible that the Collagen chains of types V and XI constitute a single collagen type with tissue specific chain combinations. Mutations in this gene are associated with Ehlers Danlos syndrome, types I and II.
• Synonyms: COL5A1