c Kit (KIT) (C-term) Rabbit Monoclonal Antibody [Clone ID: YR145]

CAT#: AM32271SU-N

c Kit (KIT) (C-term) rabbit monoclonal antibody, clone YR145, Supernatant

Specifications

Product Data

• Clone Name: YR145
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on frozen and paraffin sections: 1/25 - 1/100.
  Preparation and Pretreatment:
  1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.
  2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.
  3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
  Positive control: Breast, GIST, Skin, Testes
  Staining pattern: Cytoplasmic, Membranous
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Specificity: This antibody reacts to CD117.
• Formulation: PBS, pH 7.4
  State: Supernatant
  State: Liquid supernatant
  Stabilizer: 0,9% BSA
  Preservative: 0,09% sodium azide
• Storage:

  Store the antibody undiluted at 2-8°C.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 3815 Human
• Background:

  CD117, c-kit is a tyrosine kinase receptor found on interstitial cells of Cajal, germ cells, bone marrow stem cells, melanocytes, breast epithelium and mast cells. This receptor is found on a wide variety of tumor cells (follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, breast; malignant melanoma, endodermal sinus tumor, and small cell carcinoma) but has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi’s sarcoma, and tumors of smooth muscle origin.

• Synonyms: SCFR, KIT