c Kit (KIT) (C-term) Rabbit Monoclonal Antibody [Clone ID: YR145]

CAT#: AM32271SU-N

c Kit (KIT) (C-term) rabbit monoclonal antibody, clone YR145, Supernatant


USD 830.00

2 Weeks*

Size
    • 1 ml

Product Images

Specifications

Product Data
Clone Name YR145
Applications IHC
Recommended Dilution Immunohistochemistry on frozen and paraffin sections: 1/25 - 1/100.
Preparation and Pretreatment: 
1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C. 
2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water. 
3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
Positive control: Breast, GIST, Skin, Testes
Staining pattern: Cytoplasmic, Membranous
Reactivities Human
Host Rabbit
Isotype IgG
Clonality Monoclonal
Specificity This antibody reacts to CD117.
Formulation PBS, pH 7.4
State: Supernatant
State: Liquid supernatant
Stabilizer: 0,9% BSA
Preservative: 0,09% sodium azide
Storage

Store the antibody undiluted at 2-8°C.

Stability Shelf life: one year from despatch.

Background

CD117, c-kit is a tyrosine kinase receptor found on interstitial cells of Cajal, germ cells, bone marrow stem cells, melanocytes, breast epithelium and mast cells. This receptor is found on a wide variety of tumor cells (follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, breast; malignant melanoma, endodermal sinus tumor, and small cell carcinoma) but has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi’s sarcoma, and tumors of smooth muscle origin.

Synonyms SCFR, KIT
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.