Lipid A Mouse Monoclonal Antibody [Clone ID: 26-5]

CAT#: AM32312SU-N

Lipid A mouse monoclonal antibody, clone 26-5, Purified

Specifications

Product Data

• Clone Name: 26-5
• Applications: AGG, ELISA
• Recommended Dilution: ELISA.
  Agglutination assays.
  Recommended Dilutions:
  For ELISA use a dilution of 1/10 in PBS containing 4% BSA and 0.05% Tween.
  For Agglutination Tests: The antibody should be used undiluted.
  For ELISA prepare bacteria suspension in PBS (5x10⁸ bact. /ml). Coat ELISA plate with 100 μl/well for 1 h. at 37°C, store overnight at 4°C and wash 5x with tap water with 0.05% Tween (TT). Incubate with diluted antibody, 1 h. at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-Mouse Ig Enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density.
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Specificity: This Monoclonal antibody is reactive with Lipid A using ELISA.
• Formulation: PBS
  State: Purified
  State: Liquid (0.2 µm filtered) purified Ig fraction
  Stabilizer: 0.1% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Storage:

  Store the antibody undiluted at 2-8°C.

• Stability: Shelf life: one year from despatch.

• Background: Lipid A is part of a Gram-negative bacterial endotoxin located at one end of the lipopolysaccharide (LPS) molecule. Lipid A is made up of two glucosamine units with attached acyl chains, and it usually contains one phosphate group on each carbohydrate. Lipid A functions to anchor the LPS to the outer membrane of a Gram-negative bacteria. The toxicity of Gram-negative bacteria is due to Lipid A since this is what the human immune system recognizes, though this recognition is also critical for the onset of immune responses to Gram-negative infection and for the subsequent successful fight against the infection. Lipid A may play a role in to activating cells via Toll-like receptor 4 (TLR4), MD-2, and CD14 on the cell surface. When present in the body at high concentrations during a Gram-negative bacterial infection, Lipid A can cause shock and death because it is such a potent immune system activator.