HLA-DRA Mouse Monoclonal Antibody [Clone ID: IPO-10]

CAT#: AM32359PU-S

HLA-DRA mouse monoclonal antibody, clone IPO-10, Purified


USD 430.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name IPO-10
Applications FC, IF, IHC
Recommended Dilution ELISA: Use BSA free Antibody for coating.
Flow Cytometry:
 0.5-1 µg/106 cells. 
Immunofluorescence: 0.5-1 µg/ml.
Positive Control: Ramos, Daudi or HuT78 cells, Tonsil or lymph node.
Reactivities Human, Monkey
Host Mouse
Isotype IgG3
Clonality Monoclonal
Immunogen Spleen cells of a patient with hairy cell leukemia (Daudi cells).
Specificity MAb IPO-10 defines the antigen, which appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD37 and cym. It is expressed on resting B cells and than reappears and persists in cytoplasm and on cell surface until cytoplasmic Ig appears. It is a useful antibody for diagnostics of neoplasms of B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50% of cells).
The MAb does not label T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again. This MAb labels B cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70% of B cell CLL and some B-NHL were also positive. IPO-10 reacts with AMML cells and in a majority of Hodgkins disease cases a significant percentage of affected lymph node cells were detected.
Cellular Localization:
Cell surface.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Storage Store undiluted at 2-8°C.
Stability Shelf life: one year from despatch.
Predicted Protein Size ~36 kDa
Background HLA DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of an alpha chain (36 kDa) and a beta chain (27 kDa). It is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T helper cells. They therefore have a critical role in the initiation of the immune response.
Synonyms HLA-DRA1, MHC class II antigen DRA
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.