CD34 Mouse Monoclonal Antibody [Clone ID: EQ-8D11-C1]

CAT#: AM32448PU-N

CD34 mouse monoclonal antibody, clone EQ-8D11-C1, Purified

Product Images

CGE analysis of EQ-8D11-C1: AM32448PU-N CD34 antibody was purified by Protein G Affinity Chromatography from cell culture supernatants and verified by CGE. CGE analysis of purified EQ-8D11-C1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2

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  FACS analysis of BOSC23 cells using EQ-8D11-C1 Cat.No AM32448PU-N. BOSC23 cells were transiently transfected with an expression vector encoding either CD34 (red curve) or an irrelevant protein (control transfectant: blue and green curves). Binding of EQ-8D

Specifications

Product Data

• Clone Name: EQ-8D11-C1
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: Flow Cytometry: 1.2 μg/10⁶ cells.
  Immunofluorescence: 1 μg/10⁶ cells.
  Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
• Reactivities: Sheep
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Genetic immunisation with cDNA encoding the extracellular domain of Ovine CD34
• Specificity: This antibody recognizes Ovine CD34. Other species not tested.
  Cellular Localization: Membrane.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: None
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Background: CD34 is a type I membrane protein with an apparent MW of approximately 115 kDa. It is a putative adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. CD34 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells (2). It is thought to act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components.
• Synonyms: Hematopoietic progenitor cell marker