CD74 (B-Cell Marker) Mouse Monoclonal Antibody [Clone ID: LN-2]

CAT#: AM32811PU-S

DHLAG (CD74) mouse monoclonal antibody, clone LN2, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD74 Antibody (Clone LN2).

Specifications

Product Data

• Clone Name: LN-2
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: For coating, Use Antibody without BSA.
  Western Blot: 0.5-1 µg/ml.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 1-2 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen and Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Daudi Cells or Raji Cells. Tonsil or lymph node.
• Reactivities: Baboon, Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: SU-DHL-4 lymphoma cells
• Specificity: Cellular Localization: Cell membrane, Endoplasmic reticulum membrane, Endosome, Golgi apparatus, Late endosome, Lysosome, trans-Golgi network
• Formulation: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 33-41kDa
• Gene Name: CD74 molecule
• Database Link:
  Entrez Gene 972 Human
  UniProt ID P044233
• Background: It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
• Synonyms: CLIP, DHLAG, Gamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, HLADR-gamma (HLADG), Ia antigen-associated invariant chain, la-gamma, Major histocompatibility complex class II invariant chain, MHC HLA-DR gamma chain

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Antigen processing and presentation