MART-1 / Melan-A / MLANA (Melanoma Marker) Mouse Monoclonal Antibody [Clone ID: M2-7C10]

CAT#: AM32812PU-S

MelanA (MLANA) mouse monoclonal antibody, clone M2-7C10, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human melanoma stained with MART-1 Antibody No (Clone M2-7C10). Note cytoplasmic staining of cells.

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  Formalin-Fixed, Paraffin-Embedded Human melanoma stained with MART-1 Antibody No (Clone M2-7C10). Note cytoplasmic staining of cells.

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  Formalin-Fixed, Paraffin-Embedded Human melanoma stained with MART-1 Antibody No (Clone M2-7C10). Note cytoplasmic staining of cells.

Specifications

Product Data

• Clone Name: M2-7C10
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use Antibody without BSA for Coating.
  Western Blot: 0.5-1 µg/ml.
  Immunoprecipitaion: 1-2 µg/500 µg protein lysate.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 1-2 µg/ml.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
  Positive Control: SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas.
• Reactivities: Human, Horse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant hMART-1 protein
• Specificity: Cellular Localization: Endoplasmic reticulum membrane, Golgi apparatus, Melanosome, trans-Golgi network membrane
• Formulation: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A/G
• Conjugation: Unconjugated
• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 20-22kDa (doublet)
• Gene Name: melan-A
• Database Link:
  Entrez Gene 2315 Human
  UniProt ID Q16655
• Background: This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
• Synonyms: Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA