S100A1 (Melanoma Marker) Mouse Monoclonal Antibody [Clone ID: S1/61]

CAT#: AM32830PU-T

S100A1 mouse monoclonal antibody, clone 4C4.9, Purified

Product Images

Western blot analysis of Human brain lysate using S100 Antibody at 1 ug/ml.

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  Formalin-Fixed, Paraffin-Embedded Human melanoma stained with S100 Antibody (Clone 4C4.9).

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  Formalin-Fixed, Paraffin-Embedded Human melanoma stained with S100 Antibody (Clone 4C4.9).

Specifications

Product Data

• Clone Name: S1/61
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use Antibody without BSA for Coating.
  Western Blot: 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 1-2 µg/ml.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Embedded Sections: Use C4.9 antibody at 0.25-0.5 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 1mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Recommended Positive Control: Brain, Melanoma.
• Reactivities: Bovine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified human full-length native S-100 protein conjugated to methylated BSA
• Specificity: Cellular Localization: Cytoplasm, Mitochondrion, Sarcoplasmic reticulum
• Formulation: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 10-12kDa
• Gene Name: S100 calcium binding protein A1
• Database Link:
  Entrez Gene 6271 Human
  UniProt ID P23297
• Background: S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain (i.e. in S-100A and S-100B) but not on the beta-chain of S-100 (i.e. in S-100B). This antibody can be used to localize S-100A in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, epithelial and myoepithelial cells of the breast, salivary and sweat glands. Neoplasms derived from these cells also express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.
• Synonyms: Bpb; NEF; S100 Alpha Chain; S100 Beta Chain; S100 Calcium Binding Protein A1; S100 Calcium Binding Protein B; S100 Calcium Binding Protein Beta Neural; S100-A1