Bcl-2 (Apoptosis & Follicular Lymphoma Marker) Mouse Monoclonal Antibody [Clone ID: 8C8]

CAT#: AM32832PU-T

BCL2 (41-54) mouse monoclonal antibody, clone 8C8, Purified

Product Images

Western blot analysis of Bcl-2 in Human A) HeLa, B) A549 and C) MCF7 lysate using Bcl-2 Antibody (Clone 8C8).

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  Formalin-Fixed, Paraffin-Embedded Human tonsil stained with Bcl-2 Antibody (Clone 8C8). Note cell membrane and cytoplasmic staining.

Specifications

Product Data

• Clone Name: 8C8
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: For coating, Use Antibody without BSA.
  Western blot: 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunofluorescence: 1-2 µg/ml.
  Flow Cytometry: 0.5-1µg/10⁶ cells.
  Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 min followed by cooling at RT for 20 min. Epitope unmasking in EDTA (compared to Citrate buffer) is superior.
  Positive Control: Jurkat, K562, HL-60, or HeLa Cells, Tonsil or follicular lymphomas.
• Reactivities: Human, Monkey, Porcine, Pig
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.
• Specificity: Cellular Localization: Cytoplasm, Endoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane
• Formulation: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 25-26kDa
• Gene Name: B-cell CLL/lymphoma 2
• Database Link:
  Entrez Gene 596 Human
  UniProt ID P10415
• Background: This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
• Synonyms: Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2