Renal Cell Carcinoma (Carbonic Anhydrase IX) Mouse Monoclonal Antibody [Clone ID: 66.4.C2; same as PN-15]

CAT#: AM32835PU-T

Carbonic Anhydrase IX (CA9) Marker mouse monoclonal antibody, clone 66.4.C2 (PN-15), Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human Renal Cell Carcinoma stained with RCC Antibody (Clone 66.4.C2). Note cytoplasmic staining of cells. Note cytoplasmic and cell surface staining of tumor cells.

Specifications

Product Data

• Clone Name: 66.4.C2; same as PN-15
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: For Coating Use Antibody without BSA.
  Western Blot: 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Flow Cytometry: 0.5-1 µgl/10⁶ cells.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Normal kidney or renal cell carcinoma.
• Reactivities: Equine, Human, Horse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Microsomal fraction of human renal cortical tissue homogenate
• Specificity: Cellular Localization: Cell membrane, Cell projection, Microvillus membrane, Nucleolus, Nucleus
• Formulation: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 55kDa
• Gene Name: carbonic anhydrase 9
• Database Link:
  Entrez Gene 768 Human
  UniProt ID Q16790
• Background: Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
• Synonyms: CA-IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, Carbonic dehydratase, G250, Membrane antigen MN, P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Nitrogen metabolism