CD5 Mouse Monoclonal Antibody [Clone ID: C5/473]
Specifications
Product Data | |
Clone Name | C5/473 |
Applications | FC, IF, IHC, IP, WB |
Recommended Dilution | ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 µg/106 cells. Western Blotting: 0.5-1 µg/ml. Immunofluorescence: 0.5-1 µg/ml. Immunoprecipitation: 5-10 µl/500 µg protein lysate. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Ramos, or MOLT-4 Cells, Tonsil. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Human CD5 recombinant protein. |
Specificity | Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes. Cellular Localization: Cell surface. |
Formulation | 10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide |
Concentration | 0.2 mg/ml |
Purification | Protein A/G Chromatography |
Predicted Protein Size | 67 kDa |
Gene Name | CD5 molecule |
Database Link | |
Background | CD5, a 67KDa Type I transmembrane glycoprotein, is a member of SRCR superfamily expressed specifically of thymocytes, mature T-cells and B-1a cells. CD5 has an extracellular domain with 3 SRCR domains and cytoplasmic tail is devoid of any intrinsic enzyme activity. CD5 functions as a dual receptor modulating either positive or negative co-stimulatory signals depending on the cell type and the developmental stage. It binds CD72 and provides co-stimulatory signal for T-cell growth. CD5 plays an important role in thymocyte development and also fine-tunes TCR signaling. It is associated with antigen-specific receptor complex expressed on T-cells and B-1a cells and regulates T and B-cell mediated responses. CD5 signaling involves various kinases including PI3K, Src family tyrosine kinases, CKII, Ras GAP, and members of MAPK. |
Synonyms | CD5, LEU1 |
Reference Data |
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