CD8A Mouse Monoclonal Antibody [Clone ID: C8/468]

CAT#: AM32847PU-T

CD8A mouse monoclonal antibody, clone C8/468, Purified


USD 240.00

2 Weeks*

Size
    • 20 ug

Product Images

Specifications

Product Data
Clone Name C8/468
Applications FC, IF, IHC, IP, WB
Recommended Dilution ELISA: Use BSA free Antibody for coating.
Flow Cytometry:
 0.5-1 µg/106 cells. 
Immunofluorescence: 0.5-1 µg/ml.
Western Blot:  0.5-1 µg/ml.
Immunoprecipitation: 0.5-1 µg/500 µg protein lysate. 
Immunohistochemistry on Frozen Sections:  0.5-1 µg/ml.
Immunohistochemistry on Paraffin Sections:
  0.5-1 µg/ml.
Incubation Time: 30 minutes at RT. 
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Positive Control: HuT78 or hPBL, Tonsil.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Recombinant Human CD8 protein
Specificity Recognizes Human CD8. Other species not tested. 
Cellular Localization: Cell surface.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Storage Store undiluted at 2-8°C.
Stability Shelf life: one year from despatch.
Predicted Protein Size 32 kDa
Background CD8 is a cell surface glycoprotein molecule expressed on the surface of a subset of T cells, generally the cytotoxic/suppressor cells, as well as on some natural killer cells. There are two forms of CD8, either as an alpha-alpha homodimer or alpha-beta heterodimer. The pathological role of CD8 has been observed in familial CD8 deficiency. 
A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.
Synonyms CD8 alpha chain, CD8A, MAL
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.