CD31 (PECAM1) Mouse Monoclonal Antibody [Clone ID: C31.7]

CAT#: AM32851PU-T

CD31 (PECAM1) mouse monoclonal antibody, clone C31.7, Purified

Product Images

Western blot analysis of CD31 in THP-1 lysate probed with CD31 Antibody (Clone C31.7).

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  Formalin-Fixed, Paraffin-Embedded Human Angiosarcoma stained with CD31 Antibody (Clone C31.7).

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  Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD31 Antibody (Clone C31.7).

Specifications

Product Data

• Clone Name: C31.7
• Applications: ELISA, FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA (For coating, Use Antibody without BSA).
  Western blot: 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 1-2 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
  Positive Control: Jurkat, CCRF-CEM or K-562 Cells. Tonsil, Angiosarcoma.
• Reactivities: Human, Monkey, Rabbit
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant CD31 protein.
• Specificity: This monoclonal C31.7 antibody recognizes a 100kDa glycoprotein in endothelial cells and 130kDa in platelets.
  This antibody reacts with endothelial cells in normal tissues and in benign and malignant proliferations. In cryostat sections and blood smears the antibody also stains megakaryocytes, platelets and occasionally plasma cells. It reacts weakly with mantle zone B cells, peripheral T cells, and neutrophils.
  Antibody to CD31 is of value in the study of benign and malignant vascular tumors.
  Staining for CD31 has also been used to measure angiogenesis, which reportedly predicts tumor recurrence.
  Cellular Localization: Cell surface and cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~100 kDa (endothelium) and ~130 kDa (platelets)
• Gene Name: platelet and endothelial cell adhesion molecule 1
• Database Link:
  Entrez Gene 5175 Human
• Background: CD31 (PECAM-1, or platelet endothelial cell adhesion molecule-1) is a surface protein expressed by endothelial cells, monocytes, platelets, granulocytes, and lymphocyte subsets, and makes up a large portion of endothelial intercellular junctions. CD31 is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. Reports indicate that CD31 interacts with CD38 and is involved in cellular interactions resulting in wound healing and angiogenesis. Expression of CD31 on CD4+ T lymphocytes, helps to control T lymphocyte activation, because in the absence of CD31, T cells have a greater propensity to become activated, resulting in increased susceptibility to become apoptotic. This impact of CD31 loss becomes most pronounced during severe, inflammatory, and immunological stresses such as those caused by systemic Salmonella infection. This identifies a novel role for CD31 in regulating CD4 T homeostasis.
• Synonyms: PECAM-1, EndoCAM, GPIIA'