CD45RA (Leucocyte Marker) Mouse Monoclonal Antibody [Clone ID: 158-4D3]

CAT#: AM32858PU-S

CD45 (PTPRC) (CD45RA) mouse monoclonal antibody, clone 158-4D3, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human spleen stained with CDR5RA Antibody (Clone 158-4D3).

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  Surface staining of PBMC using CD45RA Antibody (Clone 158-4D3) (red). Anti Mouse IgG FITC secondary and Mouse IgG2a isotype control (green).

Specifications

Product Data

• Clone Name: 158-4D3
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use Antibody without BSA for Coating.
  Western Blot: 0.5-1 µg/ml.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunofluorescence: 1-2 µg/ml.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Daudi, Raji, IM9, and Human lymphocytes. Human lymph nodes and tonsils.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Stimulated human leukocytes
• Specificity: Cellular Localization: Cell membrane, Membrane raft
• Formulation: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 205-220kDa
• Gene Name: protein tyrosine phosphatase, receptor type C
• Database Link:
  Entrez Gene 5788 Human
  UniProt ID P08575
• Background: Recognizes a protein of 205kDa-220kDa, identified as CD45RA [Workshop V; Code CD45.38]. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
• Synonyms: B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
• Note: The Workshop V code for clone 158-4D3 is CD45.38.