CD45 (PTPRC) (CD45RB) Mouse Monoclonal Antibody [Clone ID: PD7/26]

CAT#: AM32859PU-S

CD45 (PTPRC) (CD45RB) mouse monoclonal antibody, clone PD7/26, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human tonsil (10X) stained with CD45RB Antibody (Clone PD7/26).

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  Formalin-Fixed, Paraffin-Embedded Human tonsil (20X) stained with CD45RB Antibody (Clone PD7/26).

Specifications

Product Data

• Clone Name: PD7/26
• Applications: IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for Coating.
  Western Blot: 0.5-1 μg/ml.
  Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
  Flow Cytometry: 0.5-1 μg/10⁶ cells.
  Immunofluorescence: 0.5-1 μg/ml.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Ramos, U-698, or GA-10 cells, Tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human peripheral blood lymphocytes maintained in T cell growth factor.
• Specificity: Recognizes Human CD45RB. Other species not tested.
  Along with other CD45 antibodies, antibody from clone PD7/26 is commonly used to identify tumors of lymphoid origin.
  Cellular Localization: Cell surface and cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 180-220 kDa
• Database Link:
  Entrez Gene 5788 Human
• Background: CD45, a transmembrane multifunctional glycoprotein, is a member of Type I receptor-linked PTPase family and is expressed as multiple isoforms due to alternative splicing. Expression of these isoforms is highly regulated and shift in this expression determines T-cell activation. CD45RB consists of exon B and is predominantly expressed in naïve T-cells secreting IL-2. Its expression is low in primed/memory T cells, cells that express Th2 cytokines such as IL-4 and IL-10 and population of T-cells with regulatory function. Immunotherapy with CD45RB antibody is being widely studied in transplantation and vaccination.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200