BAX (3-16) Mouse Monoclonal Antibody [Clone ID: 2D2]

CAT#: AM32861PU-T

BAX (3-16) mouse monoclonal antibody, clone 2D2, Purified


USD 240.00

2 Weeks*

Size
    • 20 ug

Product Images

Specifications

Product Data
Clone Name 2D2
Applications FC, IF, IHC, IP, WB
Recommended Dilution ELISA: Use Free BSA Antibody for coating.
Western Blot: 
 0.5-1 µg/ml. 
Flow Cytometry:  
0.5-1 µg/106 cells.
Imunoprecipitation: 
1-2 µg/500 µg protein lysate. 
Immunofluorescence: 1-2 µg/ml.
Immunohistochemistry on Frozen and FormalinFixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.  
Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.   
Positive Control: Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin’s lymphoma.
Reactivities Human, Monkey
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen A synthetic peptide corresponding to Amino acids 3-16 of Human Bax protein
Specificity Recognizes a protein of 21kDa, identified as the Bax protein.
This Monoclonal Antibody is highly specific to Bax and does not cross react with bcl-2 alpha or bcl-X protein.
Cellular Localization: Cytoplasmic.
Negative Species: Mouse and Rat.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Affinity Chromatography on Protein A/G
Storage Store undiluted at 2-8°C.
Stability Shelf life: one year from despatch.
Predicted Protein Size 21 kDa
Gene Name Homo sapiens BCL2-associated X protein (BAX), transcript variant beta
Background The human protein Bax sits at a critical regulatory junction of apoptosis, or programmed cell death. Activated Bax changes conformation, inserts into the MOM (Mitochondrial Outer Membrane), oligomerizes, and induces MOM permeabilization, causing the release of cytochrome c, which effectively commits the cell to die. (Ma J et al., 2012). Mechanisms of membrane perforation include formation of hetero-oligomeric complexes of Bax with other pro-apoptotic proteins such as Bak, or formation of lipidic pores physically aided by mitochondrial membrane-inserted proteins (Garg P et al., 2012). Connexins play important roles in many physiological and pathological processes. In the context of apoptosis, Cx43 translocated to the mitochondria, where it interacted with Bax to initiate the mitochondrial apoptotic pathway. The 241-382 aa region of Cx43 was required for interaction with Bax. Furthermore, this region was responsible for permeabilizing mitochondrial membrane potential. Recent studies elucidate a novel mechanism of the Cx43-mediated regulation of apoptosis in pancreatic cancer (Sun Y et al., 2012).
Bax acts as a biomarker that exhibited a difference in sub-cellular localization between normal OCSE (Oral Cavity Squamous Epithelium) and OSCC (Oral Cavity Squamous Cell Carcinoma) and was also the only apoptotic protein significantly associated with prognosis. The translocation of Bax from the nucleus to the cytoplasm in OSCC is consistent with increased Bax function at the mitochondria, leading to improved sensitivity to radiotherapy-induced apoptosis in tumours with elevated Bax expression. Bax antibody can be used to study the intracellular redistribution of Bax protein upon induction of apoptosis and its unique subcellular localization. This product can also be used in immunoblot analysis to estimate variations in the expression of specific proteins involved in apoptosis signaling (Bose P et al., 2012).
Synonyms Apoptosis regulator BAX, BCL2L4, Bcl2-L-4
Reference Data
Protein Families Druggable Genome, Transmembrane
Protein Pathways Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Huntington's disease, Neurotrophin signaling pathway, p53 signaling pathway, Pathways in cancer, Prion diseases

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.