Bax (Apoptosis Marker) Mouse Monoclonal Antibody [Clone ID: 2D2]

CAT#: AM32861PU-T

BAX (3-16) mouse monoclonal antibody, clone 2D2, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Hodgkin's lymphoma stained with Bax Antibody (Clone 2D2).

Specifications

Product Data

• Clone Name: 2D2
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use Free BSA Antibody for coating.
  Western Blot: 0.5-1 µg/ml.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Imunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunofluorescence: 1-2 µg/ml.
  Immunohistochemistry on Frozen and FormalinFixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin’s lymphoma.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.
• Specificity: Cellular Localization: Cytoplasm, Mitochondrion outer membrane
• Formulation: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A/G
• Conjugation: Unconjugated
• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 21kDa
• Gene Name: BCL2 associated X protein
• Database Link:
  Entrez Gene 718948 MonkeyEntrez Gene 581 Human
  UniProt ID Q07812
• Background: Recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
• Synonyms: Apoptosis regulator BAX cytoplasmic isoform beta, Apoptosis regulator BAX membrane isoform alpha, Bax isoform psi, Bax protein cytoplasmic isoform delta, Bax protein cytoplasmic isoform gamma, Bax zeta, Bcl-2-like protein 4, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, BCL2L4

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Huntington's disease, Neurotrophin signaling pathway, p53 signaling pathway, Pathways in cancer, Prion diseases