Cd55 Rat Monoclonal Antibody [Clone ID: 3D5]

CAT#: AM32951FC-N

Cd55 rat monoclonal antibody, clone 3D5, FITC

Specifications

Product Data

• Clone Name: 3D5
• Applications: FC, WB
• Recommended Dilution: Flow Cytometry.
  Western blot.
  The typical starting working dilution is 1/50.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: NRK cells expressing transmembrane-anchored Mouse DAF
• Specificity: The monoclonal antibody 3D5 recognizes complement decay accelerating factor (DAF), also designated as CD55.
• Formulation: PBS
  Label: FITC
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Conjugation: FITC
• Database Link:
  Entrez Gene 13136 Mouse
• Background: Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Mouse DAF (CD55) is a 60 kDa transmembrane protein that binds C3b and C4b to inhibit formation and half-life of the C3 convertases. DAF is broadly distributed among cells in contact with serum, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells. Together with other complement regulators DAF protects self cells from autologous complementmediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point.
• Synonyms: CR; CROM; DAF; TC