Epcam Rat Monoclonal Antibody [Clone ID: G8.8]

CAT#: AM33039PU-N

Epcam rat monoclonal antibody, clone G8.8, Purified

Specifications

Product Data

• Clone Name: G8.8
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Western blotting: 1/100-1/1000.
  Flow Cytometry: 1/25–1/200.
  Immunocytochemistry.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections: 1/25–1/200 with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
  
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: This antibody is derived by fusion of X63-Ag8.653 mouse myeloma cells with spleen lymphocytes from rats repeatedly immunized with glycoconjugates from BALB/c Mouse derived medullary thymic epithelial cells.
• Specificity: The G8.8 antibody reacts with CD326/Ep-CAM (Epithelial Cell Adhesion Molecule), also known as gp-40 in the Mouse. Ep-CAM is a 40-42-kDa cell-surface glycoprotein expressed on thymic epithelial cells, thymic dendritic cells, immature thymocytes, a small subset of peripheral T lymphocytes, intestinal epithelium, kidney-collecting tubule epithelium, keratinocytes, Langerhans cells, as well as lymph node and splenic dendrititc cells.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Database Link:
  Entrez Gene 17075 Mouse
• Background: Ep-CAM is a 40 kD glycoprotein and can be detected at the basolateral membrane of the majority of epithelial tissues, where it is intricately linked with the cadherin-catenin complex and hence the fundamental WNT pathway responsible for intracellular signalling and polarity. This antigen functions as a homotypic calcium-independent cell adhesion molecule. Of particular interest, Ep-CAM appears to be overexpressed by the majority of human epithelial carcinomas, including colorectal, breast, prostate, head and neck, and hepatic carcinomas. The antigen is being used as a target for immunotherapy of human carcinomas.
  Formation of Ep-CAM-mediated adhesions has a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation.
• Synonyms: Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2, M4S1, MIC18