MUC2 Mouse Monoclonal Antibody [Clone ID: CCP58]

CAT#: AM33118PU-S

MUC2 mouse monoclonal antibody, clone CCP58, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded normal Human intestine stained with Mucin-2 Antibody (Clone CCP58).

Specifications

Product Data

• Clone Name: CCP58
• Applications: ELISA, FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use Antibody without BSA For coating.
  Western blot: 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 1-2 µg/ml.
  Immunohistochemistry on Frozen and Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris-HCl buffer, pH 10.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Colon cancer cell line LS174T, Small intestine.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of 29 amino acids, from Human MUC2 protein (MI-29) (VNTR region), coupled to KLH.
• Specificity: This Monoclonal CCP58 antibody Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2) and shows no cross-reaction with Human milk fat globule membranes, MUC1, or MUC3.
  Clone CCP58 is reported to react with colon gastric cancer cells, normal intestine, colon and salivary gland (immunoperoxidase staining), Human colon cancer cell line by Flow Cytometry and Western blotting.
  The CCP58 antibody is known to be specific to amino acids GTQTP (GlyThrGlnThrPro).
  Cellular Localization: Cytoplasmic and cell surface.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purification: Protein A/G Chromatography
• Predicted Protein Size: 520 kDa
• Gene Name: mucin 2, oligomeric mucus/gel-forming
• Database Link:
  Entrez Gene 4583 Human
• Background: This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
• Synonyms: MUC-2, MUC2, SMUC, Intestinal mucin-2