MUC2 Mouse Monoclonal Antibody [Clone ID: CCP58]

CAT#: AM33118PU-T

MUC2 mouse monoclonal antibody, clone CCP58, Purified


USD 240.00

2 Weeks*

Size
    • 20 ug

Product Images

Specifications

Product Data
Clone Name CCP58
Applications ELISA, FC, IF, IHC, IP, WB
Recommended Dilution ELISA: Use Antibody without BSA For coating.
Western blot: 0.5-1 µg/ml.
Immunoprecipitation:  1-2 µg/500 µg protein lysate. 
Flow Cytometry: 0.5-1 µg/106 cells. 
Immunofluorescence: 1-2 µg/ml.
Immunohistochemistry on Frozen and Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris-HCl buffer, pH 10.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: Colon cancer cell line LS174T, Small intestine.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen A synthetic peptide of 29 amino acids, from Human MUC2 protein (MI-29) (VNTR region), coupled to KLH.
Specificity This Monoclonal CCP58 antibody Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2) and shows no cross-reaction with Human milk fat globule membranes, MUC1, or MUC3. 
Clone CCP58 is reported to react with colon gastric cancer cells, normal intestine, colon and salivary gland (immunoperoxidase staining), Human colon cancer cell line by Flow Cytometry and Western blotting. 
The CCP58 antibody is known to be specific to amino acids GTQTP (GlyThrGlnThrPro).
Cellular Localization: Cytoplasmic and cell surface.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration 0.2 mg/ml
Purification Protein A/G Chromatography
Predicted Protein Size 520 kDa
Gene Name mucin 2, oligomeric mucus/gel-forming
Background This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
Synonyms MUC-2, MUC2, SMUC, Intestinal mucin-2
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.