IPO-38 Proliferation marker Mouse Monoclonal Antibody [Clone ID: SPM260]

CAT#: AM33249PU-T

IPO-38 Proliferation marker mouse monoclonal antibody, clone SPM260, Purified

Product Images

Formalin-paraffin Human Tonsil stained with IPO38 Antibody Cat.-No AM33249PU (Clone SPM260).

Specifications

Product Data

• Clone Name: SPM260
• Applications: FC, IF, IHC
• Recommended Dilution: Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Raji and PHA-stimulated (>12 hours) human or mouse lymphocytes. Breast and colorectal carcinomas.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Spleen cells of a patient with hairy cell leukemia.
• Specificity: Recognizes a protein of 14-16kDa, which is a novel nuclear antigen of proliferating cells. IPO-38 antigen is present in the nuclei of proliferating cells such as Hodgkin’s disease and non-Hodgkin’s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes.
  IPO-38 can be a useful marker of cell proliferation during monitoring of tumor progression.
  Cellular Localization: Nuclear.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Background: IPO-38 antigen is present in the nuclei of proliferating cells such as Hodgkin’s disease and non-Hodgkin’s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO-38 can be a useful marker of cell proliferation during monitoring of tumor progression.
• Synonyms: IPO38