CD56 Mouse Monoclonal Antibody [Clone ID: SPM128]

CAT#: AM33261PU-T

CD56 mouse monoclonal antibody, clone SPM128, Purified


USD 240.00

2 Weeks*

Size
    • 20 ug

Product Images

Specifications

Product Data
Clone Name SPM128
Applications FC, IF, IHC, IP, WB
Recommended Dilution ELISA: Use BSA free antibody for coating. 
Flow Cytometry: 0.5-1.0 µg/106 cells. 
Immunofluorescence: 1-2 µg/ml. 
Immunoprecipitation: 1-2 µg/500 µg protein lysate. 
Western Blot: 0.5-1 µg/ml. 
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Recommended Positive Control: Cerebellum, Pancreas, Neuroblastoma.
Reactivities Human, Rat, Zebrafish
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Membrane preparation of a small cell lung carcinoma.
Specificity This Monoclonal Antibody reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma).
Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
Cellular Localization: Cell Surface.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration 0.2 mg/ml
Purification Protein A/G Chromatography
Predicted Protein Size 180, 145 and 125 kDa
Background The CD56 molecule is a cell surface glycoprotein which is expressed on neuroendocrine cells, natural killer cells and a subset of T cells in the peripheral blood. Three main isoforms of CD56 exist. Neurons express the largest 180kD form, while haemopoietic cells express the 140kD isoform. 
In neuronal tissues, CD56 mediates homophilic and heterophilic adhesion and is implicated in neural development. Studies suggest that CD56 is also expressed on thyroid follicular epithelium and may play a role in autoimmune disease of the thyroid. CD56 is expressed in a range of tumours including tumours of the lung, neural derived malignancies and natural killer cell lymphomas.
Synonyms NCAM-1, N-CAM-1, NCAM
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.