GM CSF (CSF2) Rat Monoclonal Antibody [Clone ID: SPM469]

CAT#: AM33269PU-S

GM CSF (CSF2) rat monoclonal antibody, clone SPM469, Purified

Specifications

Product Data

• Clone Name: SPM469
• Applications: FC, IF, IHC, IP, NEUT, WB
• Recommended Dilution: ELISA: Use BSA free antibody for coating.
  Neutralization Studies: Use BSA and Azide free antibody.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Western Blot: 0.5-1 µg/ml.
  Immunoprecipitation: 0.5-1 μg/500 µg protein lysate.
  Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Positive Control: Lymph node and tonsil.
• Reactivities: Human, Monkey
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant human GM-CSF protein.
• Specificity: This Monoclonal antibody recognizes GM-CSF (Granulocyte/Macrophage - Colony Stimulating Factor).
  Cellular Localization: Secreted (extracellular).
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 22 kDa
• Database Link:
  Entrez Gene 1437 Human
• Background: Granulocyte/macrophage - Colony-stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.
• Synonyms: CSF2, GMCSF, Sargramostim, Molgramostin