Biotin Mouse Monoclonal Antibody [Clone ID: SPM375]

CAT#: AM33281PU-S

Biotin mouse monoclonal antibody, clone SPM375, Purified

Product Images

Formalin-Paraffin tonsil stained with Biotinylated Lambda Light Chain probe followed by Biotin Antibody AM33281PU (Clone SPM375). Note cell membrane & cytoplasmic staining.

Specifications

Product Data

• Clone Name: SPM375
• Applications: ELISA, FC, IF, IHC, ISH, WB
• Recommended Dilution: ELISA.
  Western Blotting.
  Confocal Microscopy.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  In situ Nucleic Acid Hybridization.
  Immunofluorescence: 0.5-1 µg/ml.
  Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 µg/ml for 30 minutes at RT.
  No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
  Recommended Positive Control: Biotinylated proteins in solution or on tissues.
• Reactivities: All Species
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Biotinylated Sheep immunoglobulin.
• Specificity: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of Biotin.
  This Monoclonal Antibody is highly specific to Biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for Biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-Biotin antibody may be substituted to decrease this noise.
  Cellular Localization: Depends on the target.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purification: Protein A/G Chromatography
• Predicted Protein Size: 244 kDa
• Synonyms: Vitamin B7, Vitamin H