Prolactin Receptor (hPRL Receptor) Mouse Monoclonal Antibody [Clone ID: B6.2]

CAT#: AM33294PU-S

Prolactin Receptor (PRLR) mouse monoclonal antibody, clone B6.2, Purified

Specifications

Product Data

• Clone Name: B6.2
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 0.5-1 μg/ml.
  Western Blotting: 0.25-0.5 μg/ml.
  Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
  Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C.
  Positive Control: Breast, placenta, liver or pancreas.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Semi-Purified human prolactin receptor
• Specificity: Cellular Localization: Membrane, Secreted
• Formulation: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 70kDa
• Gene Name: prolactin receptor
• Database Link:
  Entrez Gene 5618 Human
  UniProt ID P16471
• Background: It recognizes a protein of 70kDa, identified as prolactin receptor. Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor), which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription.
• Synonyms: CPRLP, hPRL receptor, hPRLrI, Lactogen receptor, OPR, Pr-1, Pr-3, PRL-R, Prolactin receptor delta 7/11, RATPRLR, Secreted prolactin binding protein, Truncated testis-specific box 1-C prolactin receptor