TGF alpha (TGFA) Mouse Monoclonal Antibody [Clone ID: 1E8-G6]

CAT#: AM33319PU-S

TGF alpha (TGFA) mouse monoclonal antibody, clone 1E8-G6, Purified

Specifications

Product Data

• Clone Name: 1E8-G6
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA Free Antibody for Coating.
  Western Blotting (Not tested).
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 1-2 µg/ml.
  Immunoprecipitation: 1-2 µg/ml.
  Immunohistochemistry on Frozen and Formalin-Paraffin Sections: 1-2 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Jurkat or Ramos cells, Heart, Kidney, Pituitary, Breast cancer, Melanoma.
• Reactivities: Human, Rabbit, Zebrafish
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: A 10-amino acid synthetic peptide (aa34-43) from Human TGF alpha
• Specificity: This Monoclonal Antibody reacts with the TGF alpha and shows no cross- reaction with EGF and the neuropeptide synenkephalin.
  The staining with this Monoclonal Antibody is completely blocked by the peptide used for raising this antibody. TGFα (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cel l proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
  Cellular Localization: Cytoplasmic and Extracellular (Secreted).
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purification: Protein A/G Chromatography
• Predicted Protein Size: ~6 kDa
• Database Link:
  Entrez Gene 7039 Human
• Synonyms: Transforming growth factor alpha, ETGF