CD8A Mouse Monoclonal Antibody [Clone ID: C8/468 + C8/144B]

CAT#: AM33320PU-S

CD8A mouse monoclonal antibody, clone C8/468 + C8/144B, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human tonsil (20x) stained with CD8 Antibody (Clone C8/144B+C8/144B). Note Cell surface staining.

Specifications

Product Data

• Clone Name: C8/468 + C8/144B
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Western Blot (Not tested): 0.5-1 µg/ml.
  Immunoprecipitation (Not tested): 0.5-1 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml.
  Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml.
  Incubation Time: 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: HuT78 or hPBL, Tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human CD8 recombinant protein (C8/468); A 13 amino acid syntheti c peptide from the C-terminal cytoplasmic domain of alpha chain of human CD8 molecule (C 8/144B).
• Specificity: Recognizes Human CD8. Other species not tested.
  Cellular Localization: Cell surface.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 32 kDa
• Database Link:
  Entrez Gene 925 Human
• Background: CD8 is a cell surface glycoprotein molecule expressed on the surface of a subset of T cells, generally the cytotoxic/suppressor cells, as well as on some natural killer cells. There are two forms of CD8, either as an alpha-alpha homodimer or alpha-beta heterodimer. The pathological role of CD8 has been observed in familial CD8 deficiency.
  A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.
• Synonyms: CD8 alpha chain, CD8A, MAL