Myogenin / Myf-4 (Skeletal Muscle Marker) Mouse Monoclonal Antibody [Clone ID: F5D]

CAT#: AM33347PU-T

Myogenin (MYOG) mouse monoclonal antibody, clone F5D, Purified

Product Images

Formalin-Paraffin Rhabdomyosarcoma stained with Myogenin Antibody (Clone F5D).

Specifications

Product Data

• Clone Name: F5D
• Applications: ELISA, FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: For coating, order Antibody without BSA.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Western Blot: 0.5-1 µg/ml.
  Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.
• Reactivities: Canine, Feline, Human, Mouse, Porcine, Rat, Dog, Pig
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat myogenin peptide (aa 73-94) followed by rat myogenin recombinant fragment (aa30-224) (Epitope aa138-158)
• Specificity: Cellular Localization: Nucleus
• Formulation: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A/G
• Conjugation: Unconjugated
• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 34kDa
• Gene Name: myogenin (myogenic factor 4)
• Database Link:
  Entrez Gene 4656 Human
  UniProt ID P15173
• Background: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80% amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor.
• Synonyms: bHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4