CD46 Mouse Monoclonal Antibody [Clone ID: 122.2]

CAT#: AM33360PU-S

CD46 mouse monoclonal antibody, clone 122.2, Purified

Product Images

FCM staining of human PBMCs using CD46 Antibody (Clone 122.2).

Specifications

Product Data

• Clone Name: 122.2
• Applications: FC, FN, IF, IHC, IP, WB
• Recommended Dilution: Functional Assays (Neutralization): Use Azide free Antibody.
  Western Blot: 0.5-1 µg/ml.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunofluorescence: 1-2 µg/ml.
  Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Recommended Positive Control: HeLa, K-562 or MOLT-4 cells, Kidney.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human CD46 protein.
• Specificity: This antibody recognizes Human CD46. Other Species not tested.
  Cellular Localization: Cell surface.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 56-66 kDa
• Gene Name: CD46 molecule
• Database Link:
  Entrez Gene 4179 Human
• Background: CD46 acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. CD46 acts as a co-stimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.
• Synonyms: TLX, MIC10