IL-6 (Interleukin-6), Mouse Rat Monoclonal Antibody [Clone ID: MP5-20F3]

CAT#: AM33372PU-S

Il6 rat monoclonal antibody, clone MP5-20F3, Purified

Specifications

Product Data

• Clone Name: MP5-20F3
• Applications: FC, IF, IHC, IP, Neutralize, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Western Blotting: 0.5-1 µg/ml.
  Neutralization Studies: Use Azide free Antibody (Reported in Litterature).
  Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT (Reported in Litterature).
  Positive Control: Mouse Heart.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Mouse IL-6 protein
• Specificity: Cellular Localization: Secreted
• Formulation: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 21kDa
• Gene Name: interleukin 6
• Database Link:
  Entrez Gene 16193 Mouse
  UniProt ID P08505
• Background: IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B-cells and T-cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T-cells, B-cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.
• Synonyms: B-cell stimulatory factor 2 (BSF-2); B cell differentiation factor; CTL differentiation factor (CDF); Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HPGF; HSF; Hybridoma growth factor Interferon beta-2; Hybridoma plasmacytoma growth factor; Interferon beta-2 (IFNB2)