Il6 Rat Monoclonal Antibody [Clone ID: MP5-20F3]

CAT#: AM33372PU-S

Il6 rat monoclonal antibody, clone MP5-20F3, Purified

Specifications

Product Data

• Clone Name: MP5-20F3
• Applications: FC, IF, IHC, IP, NEUT, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Western Blotting: 0.5-1 µg/ml.
  Neutralization Studies: Use Azide free Antibody (Reported in Litterature).
  Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT (Reported in Litterature).
  Positive Control: Mouse Heart.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Mouse IL-6 protein.
• Specificity: This antibody is specific for Mouse Interleukin 6 (IL-6).
  Clone MP5-20F3 may be used to neutralise IL-6 bioactivity in a Functional Assay (Abrams et al. 1992 and Kambayashi et al. 1995).
  Cellular Localization: Cytoplasmic and extracellular (secreted).
  Negative Species: Human, Rat.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purification: Protein A/G Chromatography
• Predicted Protein Size: 21 kDa
• Gene Name: interleukin 6
• Database Link:
  Entrez Gene 16193 Mouse
• Background: IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B-cells and T-cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T-cells, B-cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes.
• Synonyms: IL-6, Interferon beta-2, IFNB2, B-cell stimulatory factor 2, BSF-2, CDF