FLT4 Rat Monoclonal Antibody [Clone ID: 5F63]

CAT#: AM33420PU-N

FLT4 rat monoclonal antibody, clone 5F63, Purified

Specifications

Product Data

• Clone Name: 5F63
• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/400-1/800.
  Immunohistochemistry on Paraffin Sections: 1/100-1/500.
  
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2
• Clonality: Monoclonal
• Immunogen: This antibody was produced from a hybridoma (Mouse myeloma fused with spleen cells from a Rat) immunized with purified Mouse VEGFR3 extracellular domain.
• Specificity: This Monoclonal antibody recognizes Mouse VEGFR3 in Western blot.
  No cross reactivity to Mouse VEGFR1 and VEGFR2.
• Formulation: Lyophilized from PBS
  State: Purified
  State: Lyophilized purified IgG fraction of the Culture Supernatant
  Stabilizer: None
  Preservative: None
• Reconstitution Method: Restore with 500 µl of sterile water to a concentration of 0.2 mg/ml
• Purification: Affinity Chromatography on Protein G
• Storage: Prior to reconstitution store at 2-8°C for one month or at -20°C for longer.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Receptor tyrosine kinase VEGFR-3, also known as Flt-4, together with VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1), are the receptors for vascular endothelial growth factors (VEGF). The VEGFR family belongs to the class II subfamily of receptor tyrosine kinases (RTKs), containing a large extracellular region which is composed of seven Ig-like domains (D1–D7), a single transmembrane (TM) helix and cytoplasmic region with a tyrosine kinase activity. In VEGFR-3, the fifth Ig homology domain is proteolytically cleaved which results in polypeptides remaining linked by two disulfide bonds. VEGFR-3 is widely expressed on all endothelial cells in early embryogenesis, while, in adult tissues, VEGFR-3 expression disappears from the vascular endothelial cells and is observed only on the lymphatic endothelium. VEGF-C and VEGF-D activation of VEGFR-3 plays an important role in the formation of the lymphatic vessel system.
• Synonyms: VEGFR3, FLT4, VEGF Receptor 3