Shh (N-term) Rat Monoclonal Antibody [Clone ID: 6K12]

CAT#: AM33478PU-N

Shh (N-term) rat monoclonal antibody, clone 6K12, Purified

Specifications

Product Data

• Clone Name: 6K12
• Applications: IHC, NEUT, WB
• Recommended Dilution: Western Blot: 1/250-1/1000.
  Neutralization.
  Immunohistochemistry on Frozen Sections: 1/50-1/200.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2
• Clonality: Monoclonal
• Immunogen: Recombinant Mouse Sonic Hedgehog (Shh) N-Terminal fragment.
• Specificity: This antibody detects Mouse Shh with Western Blot.
• Formulation: State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 0.2 ml sterile PBS and the final concentration is 0.5 mg/ml.
• Purification: Affinity Chromatography on Protein A/G
• Database Link:
  Entrez Gene 20423 Mouse
• Background: Human Shh cDNA encodes a 462 amino acid (aa) residue (45 kDa) precursor protein with a 23 aa signal peptide. An autocatalytic cleavage reaction yields a 19 kDa (residues 24 - 197) amino-terminal fragment (Shh-N), and a 25 kDa (residues 198 - 462) carboxy-terminal domain (Shh-C). The N-terminal domain retains all known signaling capabilities, while the C-terminal domain is responsible for the intramolecular processing, acting as a cholesterol transferase that covalently transfers the cholesterol molecule to the C-terminus of Shh-N. When Shh is expressed in insect or mammalian cells, a palmitoyl group is also attached to the N-terminal cysteine of Shh-N via an amide linkage. Although the binding affinity to their receptors is not changed, lipid-modified Shh-N proteins are more potent than the unmodified proteins in cell-based assays. Other hydrophobic modifications to unmodified Shh-N, including the substitution of the N-terminal cysteine residue with two hydrophobic isoleucine residues, can also increase Shh-N potency.
• Synonyms: Sonic hedgehog protein, HHG-1