CD31 (PECAM1) Mouse Monoclonal Antibody [Clone ID: SPM122]

CAT#: AM50153PU-T

CD31 (PECAM1) mouse monoclonal antibody, clone SPM122, Purified

Product Images

Formalin-fixed, paraffin-embedded human tonsil stained with CD31 Antibody (Clone SPM122).

Specifications

Product Data

• Clone Name: SPM122
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 0.5-1 μg/ml.
  Western Blotting: 0.5-1 μg/ml.
  Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Tonsil, Angiosarcoma.
• Reactivities: Human, Monkey, Rabbit
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Membrane preparation of a spleen from a patient with hairy cell leukemia (JC/70A).
• Specificity: Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
  Cellular Localization: Cell surface and cytoplasm of endothelial cells.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~100 kDa (endothelium) and ~130 kDa (platelets)
• Gene Name: platelet and endothelial cell adhesion molecule 1
• Database Link:
  Entrez Gene 5175 Human
• Background: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation.
• Synonyms: PECAM-1, EndoCAM, GPIIA'