CD45 (PTPRC) (CD45RO) Mouse Monoclonal Antibody [Clone ID: SPM125]

CAT#: AM50158PU-T

CD45 (PTPRC) (CD45RO) mouse monoclonal antibody, clone SPM125, Purified


USD 240.00

2 Weeks*

Size
    • 20 ug

Product Images

Specifications

Product Data
Clone Name SPM125
Applications FC, IF, IHC, IP, WB
Recommended Dilution ELISA: Use BSA free Antibody for coating.
Flow Cytometry: 0.5-1 μg/million cells.
Immunofluorescence: 1-2 μg/ml.
Western Blotting: 0.5-1 μg/ml.
Immunoprecipitation: 1-2 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
Reactivities Chimpanzee, Human
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Cultured human T-cells from an IL-2-dependent T-cell line (CA1).
Specificity Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Cellular Localization: Cell surface.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Storage Store undiluted at 2-8°C.
Stability Shelf life: one year from despatch.
Predicted Protein Size 180-185 kDa
Synonyms PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.