CD45 (PTPRC) (CD45RO) Mouse Monoclonal Antibody [Clone ID: SPM125]

CAT#: AM50158PU-T

CD45 (PTPRC) (CD45RO) mouse monoclonal antibody, clone SPM125, Purified

Product Images

Formalin-paraffin human tonsil stained with CD45RO Antibody (Clone SPM125).

• 

  CD45RO expression by lymphocyte gated population of PBMC: PBMC were stained either with purified CD45RO Antibody (Clone SPM125).

Specifications

Product Data

• Clone Name: SPM125
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 1-2 μg/ml.
  Western Blotting: 0.5-1 μg/ml.
  Immunoprecipitation: 1-2 μg/500 μg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1.0 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
• Reactivities: Chimpanzee, Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Cultured human T-cells from an IL-2-dependent T-cell line (CA1).
• Specificity: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
  Cellular Localization: Cell surface.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 180-185 kDa
• Database Link:
  Entrez Gene 5788 Human
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200