Actin, Smooth Muscle (Leiomyosarcoma Marker) Mouse Monoclonal Antibody [Clone ID: SPM332]

CAT#: AM50166PU-S

alpha smooth muscle Actin (ACTA2) mouse monoclonal antibody, clone SPM332, Purified

Product Images

Formalin-paraffin leiomyosarcoma stained with Smooth Muscle Actin Antibody (Clone SPM332).

Specifications

Product Data

• Clone Name: SPM332
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 0.5-1 μg/million cells.
  Immunofluorescence: 0.5-1 μg/ml.
  Western Blotting: 0.5-1 μg/ml.
  Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
  Immunohistology (Formalin-Fixed): 0.5-1.0 μg/ml for 30 minutes at RT.
  (Staining of Formalin-Fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min.)
  Positive Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma.
• Reactivities: Baboon, Bovine, Canine, Chicken, Feline, Guinea Pig, Goat, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Cow, Dog, Pig
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.
• Specificity: Cellular Localization: Cytoplasm, Cytoskeleton
• Formulation: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 42kDa
• Gene Name: actin, alpha 2, smooth muscle, aorta
• Database Link:
  Entrez Gene 59 Human
  UniProt ID P62736
• Background: Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
• Synonyms: ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Aortic Smooth Muscle, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha 2 Actin, Alpha Actin 2, Alpha Cardiac Actin, Alpha Smooth Muscle, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46