CD5 Mouse Monoclonal Antibody [Clone ID: SPM546]

CAT#: AM50190PU-S

CD5 mouse monoclonal antibody, clone SPM546, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD5 Antibody (Clone SPM546).

Specifications

Product Data

• Clone Name: SPM546
• Applications: FC, IF, IHC
• Recommended Dilution: Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 min at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: 293T, Ramos, or MOLT-4 Cells, Tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human CD5 recombinant protein.
• Specificity: Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.
  Cellular Localization: Cell surface.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purification: Protein A/G Chromatography
• Predicted Protein Size: 67 kDa
• Gene Name: CD5 molecule
• Database Link:
  Entrez Gene 921 Human
• Synonyms: CD5, LEU1