CD2 Mouse Monoclonal Antibody [Clone ID: UMCD2]

CAT#: AM50214PU-T

CD2 mouse monoclonal antibody, clone UMCD2, Purified

Specifications

Product Data

• Clone Name: UMCD2
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Functional Studies: Use Azide free Antibody.
  Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Western Blotting: 0.5-1 µg/ml.
  Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Positive Control: 293T or Jurkat cells. Lymph node and tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human Thymocytes/Sezary T cells.
• Specificity: Recognizes CD2.
  Cellular Localization: Cell surface.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 50 kDa
• Gene Name: CD2 molecule
• Database Link:
  Entrez Gene 914 Human
• Background: CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.
• Synonyms: SRBC, Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor