CD20 (MS4A1) Mouse Monoclonal Antibody [Clone ID: B9E9]

CAT#: AM50217PU-S

CD20 (MS4A1) mouse monoclonal antibody, clone B9E9, Purified


USD 430.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name B9E9
Applications IF, IHC, IP
Recommended Dilution ELISA: Use BSA free Antibody for coating.
Functional Studies: (Use Antibody without BSA and Azide. 
Flow Cytometry: 0.5-1 µg/million cells. 
Immunofluorescence: 0.5-1 µg/ml. 
Immunoprecipitation: 0.5-1 μg/500 µg protein lysate.
Immunohistochemistry on Frozen Sections: 0.5-1.0 µg/ml for 30 min at RT.
Positive Control: Daudi, Raji, and U266, and human lymphocytes. In normal lymph nodes and tonsils, CD20 is predominantly detected on B cells in germinal centers.
Reactivities Human
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Lymphoblastoid cell line Daudi.
Specificity Recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12).
B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This Monoclonal Antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. 
Cellular Localization: Predominantly cell surface with some cytoplasmic.
Formulation 10mM PBS
State: Purified
State: Liquid purified IgG fraction from Bioreactor Concentrate
Stabilizer: 0.05% BSA
Preservative: 0.05% Sodium Azide
Concentration lot specific
Purification Protein A/G Chromatography
Storage Store undiluted at 2-8°C.
Stability Shelf life: one year from despatch.
Predicted Protein Size 33-37 kDa
Background CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
Synonyms MS4A1, Leu-16, Bp35, B-cell marker
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.