CD20 (MS4A1) Mouse Monoclonal Antibody [Clone ID: B9E9]

CAT#: AM50217PU-S

CD20 (MS4A1) mouse monoclonal antibody, clone B9E9, Purified

Specifications

Product Data

• Clone Name: B9E9
• Applications: IF, IHC, IP
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Functional Studies: (Use Antibody without BSA and Azide.
  Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Immunoprecipitation: 0.5-1 μg/500 µg protein lysate.
  Immunohistochemistry on Frozen Sections: 0.5-1.0 µg/ml for 30 min at RT.
  Positive Control: Daudi, Raji, and U266, and human lymphocytes. In normal lymph nodes and tonsils, CD20 is predominantly detected on B cells in germinal centers.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Lymphoblastoid cell line Daudi.
• Specificity: Recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12).
  B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This Monoclonal Antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods.
  Cellular Localization: Predominantly cell surface with some cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 33-37 kDa
• Database Link:
  Entrez Gene 931 Human
• Background: CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
• Synonyms: MS4A1, Leu-16, Bp35, B-cell marker