CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody [Clone ID: JC/70A]

CAT#: AM50226PU-S

CD31 (PECAM1) mouse monoclonal antibody, clone JC/70A, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human Angiosarcoma stained with CD31 Antibody (Clone JC/70A).

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  Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD31 Antibody (Clone JC/70A).

Specifications

Product Data

• Clone Name: JC/70A
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Western blot: 0.5-3 µg/ml.
  Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.
  Positive Control: Tonsil, Angiosarcoma.
• Reactivities: Human, Monkey, Rabbit
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Membrane preparation of a spleen from a patient with hairy cell leukemia
• Specificity: Cellular Localization: Cell junction, Cell membrane, Membrane raft
• Formulation: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~100kDa (endothelium) and ~130kDa (platelets)&apos
• Gene Name: platelet and endothelial cell adhesion molecule 1
• Database Link:
  Entrez Gene 5175 Human
  UniProt ID P16284
• Background: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
• Synonyms: EndoCAM; GPIIA'; PECA1; Platelet Endothelial Cell Adhesion Molecule 1