CD45 (PTPRC) (CD45RB) Mouse Monoclonal Antibody [Clone ID: BRA-11; same as BRA-11G]

CAT#: AM50232PU-T

CD45 (PTPRC) (CD45RB) mouse monoclonal antibody, clone BRA-11; same as BRA-11G, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human tonsil stained with CD45RB Antibody (Clone BRA-11).

Specifications

Product Data

• Clone Name: BRA-11; same as BRA-11G
• Applications: IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Western Blotting: 0.5-1.0 µg/ml.
  Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1.0 µg/ml for 30 minutes at RT.
  (taining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Ramos, U-698, or GA-10 cells, Tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Non-T, non-B CALLA positive ALL cell line REH (Leucocyte Workshop IV and V).
• Specificity: Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
  Cellular Localization: Cell surface and cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 180-220 kDa
• Database Link:
  Entrez Gene 5788 Human
• Background: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200