CD56 (NCAM1) Mouse Monoclonal Antibody [Clone ID: 123A8; same 56C04]

CAT#: AM50239PU-S

CD56 (NCAM1) mouse monoclonal antibody, clone 123A8; same 56C04, Purified

Product Images

Formalin-paraffin human colon ganglion stained with CD56 Antibody (Clone 123A8).

Specifications

Product Data

• Clone Name: 123A8; same 56C04
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for Coating.
  Western Blot (Not tested): 0.5-1 μg/ml.
  Immunoprecipitation (Not tested): 1-2 μg/500 μg protein lysate.
  Flow Cytometry (Not tested): 0.5-1 μg/10⁶ cells.
  Immunofluorescence: 1-2 μg/ml.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Cerebellum, Pancreas, Neuroblastoma.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Membrane preparation of a small cell lung carcinoma.
• Specificity: This Monoclonal Antibody reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species.
  Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma).
  Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
  Cellular Localization: Cell surface.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 180, 145 and 125 kDa
• Database Link:
  Entrez Gene 4684 Human
• Synonyms: NCAM-1, N-CAM-1, NCAM